{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Charles Girardot"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13694"],"description":["To determine glycolytic flux-responsive genes in mouse presomitic mesoderm (PSM) cells, RNA sequencing (single-end) was performed using tailbud tissues isolated from wild-type and cytoPFKFB3 PSM explants cultured in different glucose conditions. Smart-seq2 protocol was used, and each condition includes three biological replicates.  This study is complementary to ENA Project PRJEB55095."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - single-end sequencing on illumina NextSeq 500","Growth Protocol - Conditional cytoPFKFB3 mice (Miyazawa et al., eLIFE, 2022) were crossed with Hprt-Cre mice, and females were dissected 10 days after a detection of vaginal plug. Isolated PSM tissues were cultured for three hours either in 0.5 mM or 2.0 mM glucose condition.","Library Construction - RNA-seq libraries were prepared following the Smart-seq2 described in Picelli et al., Nat Protoc, 2014"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Charles Girardot","Hidenobu Miyazawa"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of PSM tailbud tissues isolated from wild-type and cytoPFKFB3 mouse embryos cultured in different glucose concentration","description":"To determine glycolytic flux-responsive genes in mouse presomitic mesoderm (PSM) cells, RNA sequencing (single-end) was performed using tailbud tissues isolated from wild-type and cytoPFKFB3 PSM explants cultured in different glucose conditions. Smart-seq2 protocol was used, and each condition includes three biological replicates.  This study is complementary to ENA Project PRJEB55095.","dates":{"release":"2025-08-06T00:00:00Z","modification":"2025-08-07T00:02:47.333Z","creation":"2024-01-04T14:21:56.405Z"},"accession":"E-MTAB-13694","cross_references":{"ENA":["ERP156461"],"EFO":["EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}