biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsCharles GirardotNextSeq 500RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13694To determine glycolytic flux-responsive genes in mouse presomitic mesoderm (PSM) cells, RNA sequencing (single-end) was performed using tailbud tissues isolated from wild-type and cytoPFKFB3 PSM explants cultured in different glucose conditions. Smart-seq2 protocol was used, and each condition includes three biological replicates.  This study is complementary to ENA Project PRJEB55095.biostudies-arrayexpressSequencing - single-end sequencing on illumina NextSeq 500Growth Protocol - Conditional cytoPFKFB3 mice (Miyazawa et al., eLIFE, 2022) were crossed with Hprt-Cre mice, and females were dissected 10 days after a detection of vaginal plug. Isolated PSM tissues were cultured for three hours either in 0.5 mM or 2.0 mM glucose condition.Library Construction - RNA-seq libraries were prepared following the Smart-seq2 described in Picelli et al., Nat Protoc, 2014OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesCharles GirardotHidenobu MiyazawafalseRNA-seq of PSM tailbud tissues isolated from wild-type and cytoPFKFB3 mouse embryos cultured in different glucose concentrationTo determine glycolytic flux-responsive genes in mouse presomitic mesoderm (PSM) cells, RNA sequencing (single-end) was performed using tailbud tissues isolated from wild-type and cytoPFKFB3 PSM explants cultured in different glucose conditions. Smart-seq2 protocol was used, and each condition includes three biological replicates.  This study is complementary to ENA Project PRJEB55095.2025-08-06T00:00:00Z2025-08-07T00:02:47.333Z2024-01-04T14:21:56.405ZE-MTAB-13694ERP156461EFO_0004170EFO_0005518EFO_0003738EFO_0004184