biostudies-arrayexpressDrosophila melanogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13735RNA Seq analysis of total RNA from adult ovaries to determine differential gene expression between the following genotypes: w[1118] control strain, homozygous tox4 null allele, with or without GFP-tox4 transgenic rescue, homozygous PNUTS null allele with transgenic constructs disrupting PP1-binding or tox4-binding, each in triplicate. Read depth was approx. 45-55M reads/replicate.biostudies-arrayexpressGrowth Protocol - Flies were grown at 25°C on standard media containing maize, sugar, yeast, agar and anti-fungal agents.Library Construction - RNA-sequencing libraries were constructed using the Stranded Total RNA Prep. Ligation with Ribo-Zero Plus kit (Illumina, Inc.) according to the manufacturer’s protocol, except with a hybridization step using customised oligonucleotide probes for depletion of Drosophila rRNA.Sequencing - 159 bp paired-end reads from biological triplicates for each condition were generated using the NovaSeq6000 system (Illumina).Sample Collection - Ovaries were dissected in 1 x PBS and then transferred into TRIzol reagent.Nucleic Acid Extraction - RNA was extracted from 10 pairs of ovary/sample. Total RNA was extracted according to the manufacturer’s instructions and RNA was stored in RNAse free water. Quantity and quality of RNA was quantified using Nanodrop ND-2000 (Thermo Fisher Scientific) and Agilent TapeStation system (Agilent).MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNADrosophila melanogasterERP156843Ian DonaldsonDaimark BennettfalseGenome-wide study of the role of Tox4 on gene expression in adult Drosophila ovariesRNA Seq analysis of total RNA from adult ovaries to determine differential gene expression between the following genotypes: w[1118] control strain, homozygous tox4 null allele, with or without GFP-tox4 transgenic rescue, homozygous PNUTS null allele with transgenic constructs disrupting PP1-binding or tox4-binding, each in triplicate. Read depth was approx. 45-55M reads/replicate.2025-05-06T00:00:00Z2026-06-05T19:05:30.77Z2024-01-24T12:37:21.421ZE-MTAB-13735ERP156843EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184