biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNares TrakooljulIllumina HiSeq 2500RNA-seq of coding RNASus scrofaSus scrofahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13759We have reported the GRAla610Val substitution mutation in pigs (Murani et.al., 2012; DOI: 10.1371/journal.pone.0045518). This experiment aims to investigate the amygdala transcriptomic profile of pigs with different genotypes at the GRAla610Val under unchallenged/resting environmental condition of both male and female pigs. The transcriptome signatures and differentially expressed genes associated with the genotypes may provide insights into mechanisms modulating glucocorticoid sensitivity and better understanding of phenotypic consequences as well as sex-specific effects of the GRAla610Val substitution.biostudies-arrayexpressSequencing - Multiplexed DNA libraries were sequenced for 2x101bp paired-end reads in high-output mode according to the manufacturer’s recommendation using an Illumina HiSeq 2500 instrument at the Institute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.Nucleic Acid Extraction - Total RNA was isolated using TRI reagent (Sigma-Aldrich, Taufkirchen, Germany). DNA traces were removed by DNaseI-treatment (Baseline-Zero DNase; Biozym, Hessisch Oldendorf, Germany), and the RNA was cleaned-up using the RNA Clean&Concentrator-25 Kit (Zymo Research, Freiburg, Germany). RNA quantity was determined using a Qubit fluorometer (ThermoFisher Scientific, Germany), and integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).Library Construction - 2 µg of total RNA with RIN > 8 was used for library preparation using a TruSeq Stranded mRNA Kit with 11 cycles of PCR-amplification according to the manufacture’s recommendation (Illumina).Sample Collection - The animals were anesthetized by an intravenous (i.v.) administration of a combination of ketamine (Ursotamin; Serum-Werk Bernburg, Bernburg, Germany) and azaperone (Stresnil; Janssen-Cilag, Neuss, Germany), and euthanized by an i.v. administration of T61 (Intervet, Unterschleißheim, Germany). Tissue samples were quickly dissected (<10min), frozen in liquid nitrogen, and stored at -80ºC. The experimental protocol (7221.3-1-024/16) has been approved by the Animal Care Committee of the Research Institute for Farm Animal Biology and the State Mecklenburg-Western Pomerania (Landesamt für Landwirtschaft, Lebensmittelsicherheit und Fischerei).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesNares TrakooljulEduard MuranifalseTranscriptomic profiling of amygdala of pigs carrying different genotypes at the gain-of-function mutation of glucocorticoid receptorWe have reported the GRAla610Val substitution mutation in pigs (Murani et.al., 2012; DOI: 10.1371/journal.pone.0045518). This experiment aims to investigate the amygdala transcriptomic profile of pigs with different genotypes at the GRAla610Val under unchallenged/resting environmental condition of both male and female pigs. The transcriptome signatures and differentially expressed genes associated with the genotypes may provide insights into mechanisms modulating glucocorticoid sensitivity and better understanding of phenotypic consequences as well as sex-specific effects of the GRAla610Val substitution.2026-09-02T00:00:00Z2026-09-02T11:43:15.212Z2024-02-02T10:59:38.097ZE-MTAB-13759ERP157065E-MTAB-7494E-MTAB-7495E-MTAB-9098EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184