{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Amit Dutt"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["DNA-seq"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13774"],"description":["The study involves targeted sequencing of 30 orphan FFPE tumors obtained from anaplastic thyroid carcinoma patients of Indian origin. With this, we aim to describe the mutation profile of this specific subset of anaplastic thyroid cancer patients. This knowledge will further allow us to gain an insight into genomic alterations prevalent in Indian anaplastic thyroid carcinoma."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - The FFPE tumor tissues were collected from ACTREC-TMC","Library Construction - The service provider's standardized 1000 gene panel known as Indegene panel have been used and targeted DNA sequencing was done on 30 FFPE samples of anaplastic thyroid cancer","Sequencing - 30 samples' targeted sequencing libraries were put into the Novaseq 6000 platform at 4basecare Pvt. Ltd., Bangalore, for 2 X 150 bp paired-end sequencing","Nucleic Acid Extraction - The 30 FFPE orphan tumor samples has been subjected to microtome-sectioning where ~5-6 sections of 10 um has been taken based on the tissue abundance for DNA extraction. The DNA extraction from has been performed according to standard protocol of Qiagen's DNeasy FFPE tissue kits. The DNA concentration was measured using Nanodrop 2000c spectrophotometer."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Amit Dutt"],"additional_accession":[]},"is_claimable":false,"name":"Targeted sequencing of anaplastic thyroid cancer genome of Indian origin","description":"The study involves targeted sequencing of 30 orphan FFPE tumors obtained from anaplastic thyroid carcinoma patients of Indian origin. With this, we aim to describe the mutation profile of this specific subset of anaplastic thyroid cancer patients. This knowledge will further allow us to gain an insight into genomic alterations prevalent in Indian anaplastic thyroid carcinoma.","dates":{"release":"2025-07-01T00:00:00Z","modification":"2024-02-08T21:20:43.599Z","creation":"2024-02-08T21:20:43.599Z"},"accession":"E-MTAB-13774","cross_references":{"ENA":["ERP157208"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}