{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13785"],"description":["Same iPSC line was differentiated according to two protocols for comparison (n=6). Protocol 1 (AC) is a novel protocol while Protocol 2 (AW) is a comparator protocol. At the end of both protocols, cell lysates were collected, RNA extracted and analysed by bulk mRNA-sequencing targeting 50 million paired-end 150bp reads per sample."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Illumina NovaSeq 6000 Sequencing System, 150bp, paired-end. Performed by Novogene Ltd.","Sample Collection - Cells were washed with PBS 1x and then lysed with the lysis buffer (see extraction kit details)","Nucleic Acid Extraction - RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit following manufacturer’s instructions (Cat no. RTN70 - Sigma Aldrich)","Library Construction - PolyA enrichment of mRNA, RT first-strand cDNA library with ligation of sequencing adapters"],"figure_sub":["MINSEQE Score","Assays and Data","Processed Data","organisation","MAGE-TAB Files"],"data_protocol":["Data Transformation - fastq files aligned with the reference genome to create BAM files (STARalign v2.6.1d). Raw transcript counts quantified using FeatureCounts (v1.5.0-p3)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"additional_accession":["E-MTAB-13799","ERP157289"],"pubmed_authors":["William Dalleywater"]},"is_claimable":false,"name":"RNA-seq of two iPSC intestinal differentiation protocols","description":"Same iPSC line was differentiated according to two protocols for comparison (n=6). Protocol 1 (AC) is a novel protocol while Protocol 2 (AW) is a comparator protocol. At the end of both protocols, cell lysates were collected, RNA extracted and analysed by bulk mRNA-sequencing targeting 50 million paired-end 150bp reads per sample.","dates":{"release":"2026-03-03T00:00:00Z","modification":"2026-03-03T02:03:04.929Z","creation":"2024-02-09T10:50:30.845Z"},"accession":"E-MTAB-13785","cross_references":{"ENA":["ERP157289"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}