biostudies-arrayexpressHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13785Same iPSC line was differentiated according to two protocols for comparison (n=6). Protocol 1 (AC) is a novel protocol while Protocol 2 (AW) is a comparator protocol. At the end of both protocols, cell lysates were collected, RNA extracted and analysed by bulk mRNA-sequencing targeting 50 million paired-end 150bp reads per sample.biostudies-arrayexpressSequencing - Illumina NovaSeq 6000 Sequencing System, 150bp, paired-end. Performed by Novogene Ltd.Sample Collection - Cells were washed with PBS 1x and then lysed with the lysis buffer (see extraction kit details)Nucleic Acid Extraction - RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit following manufacturer’s instructions (Cat no. RTN70 - Sigma Aldrich)Library Construction - PolyA enrichment of mRNA, RT first-strand cDNA library with ligation of sequencing adaptersMINSEQE ScoreAssays and DataProcessed DataorganisationMAGE-TAB FilesData Transformation - fastq files aligned with the reference genome to create BAM files (STARalign v2.6.1d). Raw transcript counts quantified using FeatureCounts (v1.5.0-p3).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensE-MTAB-13799ERP157289William DalleywaterfalseRNA-seq of two iPSC intestinal differentiation protocolsSame iPSC line was differentiated according to two protocols for comparison (n=6). Protocol 1 (AC) is a novel protocol while Protocol 2 (AW) is a comparator protocol. At the end of both protocols, cell lysates were collected, RNA extracted and analysed by bulk mRNA-sequencing targeting 50 million paired-end 150bp reads per sample.2026-03-03T00:00:00Z2026-03-03T02:03:04.929Z2024-02-09T10:50:30.845ZE-MTAB-13785ERP157289EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184