{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13799"],"description":["Induced pluripotent stem cells were differentiated to intestinal lineages using a novel protocol, which results in co-differentiation of epithelial (endoderm) and non-epithelial (mesoderm) populations. Cells were separated at the end of the protocol using MACS based on EpCAM expression (EpCAM+ epithelial cells and EpCAM- non-epithelial cells). RNA was extracted and the whole transcriptome was profiled by RNA-seq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Illumina NovaSeq 6000 Sequencing System, 150bp, paired-end. Performed by Novogene Ltd.","Sample Collection - Cells were washed with PBS 1x and then lysed with the lysis buffer (see extraction kit details)","Nucleic Acid Extraction - RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit following manufacturer’s instructions (Cat no. RTN70 - Sigma Aldrich)","Library Construction - PolyA enrichment of mRNA, RT first-strand cDNA library with ligation of sequencing adapters"],"figure_sub":["MINSEQE Score","Assays and Data","Processed Data","organisation","MAGE-TAB Files"],"data_protocol":["Data Transformation - fastq files aligned with the reference genome to create BAM files (STARalign v2.6.1d). Raw transcript counts quantified using FeatureCounts (v1.5.0-p3)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"additional_accession":["E-MTAB-13803","E-MTAB-13785"],"pubmed_authors":["William Dalleywater"]},"is_claimable":false,"name":"Comparison of epithelial (EpCAM+) and non-epithelial (EpCAM-) intestinal populations derived from induced pluripotent stem cells using a novel protocol","description":"Induced pluripotent stem cells were differentiated to intestinal lineages using a novel protocol, which results in co-differentiation of epithelial (endoderm) and non-epithelial (mesoderm) populations. Cells were separated at the end of the protocol using MACS based on EpCAM expression (EpCAM+ epithelial cells and EpCAM- non-epithelial cells). RNA was extracted and the whole transcriptome was profiled by RNA-seq.","dates":{"release":"2026-03-03T00:00:00Z","modification":"2026-03-03T02:03:04.78Z","creation":"2024-02-12T22:43:57.602Z"},"accession":"E-MTAB-13799","cross_references":{"ENA":["ERP157410"],"Biostudies":["E-MTAB-13785"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}