biostudies-arrayexpressHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13799Induced pluripotent stem cells were differentiated to intestinal lineages using a novel protocol, which results in co-differentiation of epithelial (endoderm) and non-epithelial (mesoderm) populations. Cells were separated at the end of the protocol using MACS based on EpCAM expression (EpCAM+ epithelial cells and EpCAM- non-epithelial cells). RNA was extracted and the whole transcriptome was profiled by RNA-seq.biostudies-arrayexpressSequencing - Illumina NovaSeq 6000 Sequencing System, 150bp, paired-end. Performed by Novogene Ltd.Sample Collection - Cells were washed with PBS 1x and then lysed with the lysis buffer (see extraction kit details)Nucleic Acid Extraction - RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit following manufacturer’s instructions (Cat no. RTN70 - Sigma Aldrich)Library Construction - PolyA enrichment of mRNA, RT first-strand cDNA library with ligation of sequencing adaptersMINSEQE ScoreAssays and DataProcessed DataorganisationMAGE-TAB FilesData Transformation - fastq files aligned with the reference genome to create BAM files (STARalign v2.6.1d). Raw transcript counts quantified using FeatureCounts (v1.5.0-p3).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensE-MTAB-13803E-MTAB-13785William DalleywaterfalseComparison of epithelial (EpCAM+) and non-epithelial (EpCAM-) intestinal populations derived from induced pluripotent stem cells using a novel protocolInduced pluripotent stem cells were differentiated to intestinal lineages using a novel protocol, which results in co-differentiation of epithelial (endoderm) and non-epithelial (mesoderm) populations. Cells were separated at the end of the protocol using MACS based on EpCAM expression (EpCAM+ epithelial cells and EpCAM- non-epithelial cells). RNA was extracted and the whole transcriptome was profiled by RNA-seq.2026-03-03T00:00:00Z2026-03-03T02:03:04.78Z2024-02-12T22:43:57.602ZE-MTAB-13799ERP157410E-MTAB-13785EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184