biostudies-arrayexpressPatrick NylundHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13813Here we investigated the molecular response DNMT- and G9a inhibition in multiple myeloma.biostudies-arrayexpressLibrary Construction - Sequencing libraries were prepared from 100ng total RNA using the Illumina Stranded Total RNA library preparation kit with Ribo-Zero Plus treatment (cat# 20040525/20040529, Illumina Inc.)Sample Collection - INA-6, OPM2 and KMS-28PE were selected for combinatorial treatment response analysis. INA-6 and KMS28-PE cells were seeded at 100.000 cells/ml while OPM2 cells were seeded at 200.000 cells/mL 24h prior treatment with H2O and DMSO (Sigma-Aldrich) or 50nM of AZA (Abcam) and 5μM or 10μM of A366 (Tocris Bio-techne). AZA treatment was administrated every day while A366 treatment was added every 3 days. Media and reagent refreshment was performed every 3 days. All experiments were performed in three independent cell batches per cell line.Nucleic Acid Extraction - RNA was extracted using the RNeasy Micro Kit (Qiagen; Hilden; Germany #74004) as described by the manufacturer.Sequencing - Paired-end 150bp read length, NovaSeq 6000 system, S4 flowcell and v1.5 sequencing chemistry.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - The sequencing data from the combinatorial treated INA-6, KMS-28PE and OPM2 MM cell lines were analysed using the nf-core RNAseq pipeline (https://doi.org/10.5281/zenodo.3503887) in version 3.4, utilizing default parameters for paired-end analysis. Differential expression analysis was done by DESeq2 workflow as described: http://master.bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.htmlMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of total RNAHomo sapiensPatrick NylundBerta Garrido-ZabalafalseRNA-seq experiment of multiple myeloma cells under different treatment conditions against epigenetic modifiersHere we investigated the molecular response DNMT- and G9a inhibition in multiple myeloma.2025-12-31T00:00:00Z2025-12-31T02:02:13.02Z2024-02-28T21:19:02.117ZE-MTAB-13813ERP157492EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184