{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13877"],"description":["To identify candidate predictive markers, our study used two mouse models: one of them consists in Balb/c adult male mice exhibiting interindividual variability in obesity predisposition. A HFD challenge of 18 weeks allow to classify each mice according to its susceptibility to obesity. Transcriptomic analysis was performed on biopsies of White adipose tissues sample from 27 mice before the HFD Challenge."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Labeling - Gene expression profiles were performed at the GeT‐TRiX facility (GénoToul, Génopole Toulouse Midi-Pyrénées) using Agilent Sureprint G3 Mouse GE v2 microarrays (8x60K, design 074809) following the manufacturer's instructions. For each sample, Cyanine-3 (Cy3) labeled cRNA was prepared from 50 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer's instructions, followed by Agencourt RNAClean XP (Agencourt Bioscience Corporation, Beverly, Massachusetts). Dye incorporation and cRNA yield were checked using DropsenseTM 96 UV/VIS droplet reader (Trinean, Belgium).","Scaning - Immediately after washing, the slides were scanned on Agilent G2505C Microarray Scanner using Agilent Scan Control A.8.5.1 software and fluorescence signal extracted using Agilent Feature Extraction software v10.10.1.1 with default parameters.","Nucleic Acid Extraction - Total RNA was prepared using the RNeasy minikit (Qiagen)","Sample Collection - For perigonadal WAT biopsy, mice were placed on an heating-pad under isoflurane anesthesia using a nose cone. At the end of experiments, mice were euthanized by cervical dislocation under isoflurane anesthesia. Perigonadal WAT was collected prior to immediate freezing in liquid nitrogen and stored at −80 ◦C until analyses.","Hybridization - 600 ng of Cy3-labelled cRNA were hybridized on the microarray slides following the manufacturer’s instructions."],"figure_sub":["MIAME Score","Raw Data","Assays and Data","Processed Data","organisation","MAGE-TAB Files","Array Designs"],"data_protocol":["Data Transformation - For mouse microarray data, differential analysis between the three groups (R, I, P) was performed with the limma package (v3.54.2) through the lmFit() and eBayes() functions [30]. A selection threshold was applied at 0.05 on the Benjamini-Hochberg adjusted p-values."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"study_type":["transcription profiling by array"],"species":["Mus musculus"],"pubmed_title":["Identification of a specific set of genes predicting obesity before phenotype appearance"],"pubmed_authors":["Céline Jousse, Laurent Parry, Gwendal Cueff, Marion Brandolini-Bunlon, Jérémy Tournayre, Alain Bruhat, Anne-Catherine Maurin, Cyrielle Vituret, Julien Averous, Yuki Muranishi, and Pierre Fafournoux","Gwendal Cueff","Céline Jousse"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic analysis of WAT from male mice innately exhibiting various susceptibility to diet-induced-obesity: samples from mice at T0","description":"To identify candidate predictive markers, our study used two mouse models: one of them consists in Balb/c adult male mice exhibiting interindividual variability in obesity predisposition. A HFD challenge of 18 weeks allow to classify each mice according to its susceptibility to obesity. Transcriptomic analysis was performed on biopsies of White adipose tissues sample from 27 mice before the HFD Challenge.","dates":{"release":"2025-04-22T00:00:00Z","modification":"2026-05-27T13:40:25.361Z","creation":"2024-03-05T12:13:44.225Z"},"accession":"E-MTAB-13877","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0005518","EFO_0003816","EFO_0003815"]}}