{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["veronica manicardi"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of total RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13925"],"description":["The aim of this experiment was to identify NEAT1-dependent transcriptional program in multiple myeloma. AMO-1 cell line was silenced for NEAT1 expression using antisense oligonucleotide gapmeR (g#N1_E). Not-targeting gapmeR was used as control (g#SCR). RNA was collected 96 hours after gapmeR delivery."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - AMO-1 were cultured in RPMI supplemented with 10% FBS and 1% Penicillin-Streptomycin.","Nucleic Acid Extraction - Total RNA was extracted by TRIzol reagent according to the manufacturer’s instructions.","Sequencing - Sequencing was performed by Illumina Nextseq 500 instrument (High output kit v2, 2x150 cycles).","Sample Collection - Cells were collected after 96 hours from gymnotic delivery.","Library Construction - RNAseq libraries were prepared by Illumina® Stranded Total RNA Prep kit starting from 500 ng of RNA. RNA quality was assessed by 4200 Tapestation using RNA screen tape kit protocol.","Sample Treatment - Cells were seeded at 5x10^4 cells/ml and concurrently treated with naked gapmeR (g#N1_E and g#SCR)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["veronica manicardi"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of AMO-1 multiple myeloma cell line silenced for lncRNA NEAT1 expression and relative control condition","description":"The aim of this experiment was to identify NEAT1-dependent transcriptional program in multiple myeloma. AMO-1 cell line was silenced for NEAT1 expression using antisense oligonucleotide gapmeR (g#N1_E). Not-targeting gapmeR was used as control (g#SCR). RNA was collected 96 hours after gapmeR delivery.","dates":{"release":"2026-03-06T00:00:00Z","modification":"2026-03-06T09:48:27.74Z","creation":"2024-03-19T18:55:29.605Z"},"accession":"E-MTAB-13925","cross_references":{"ENA":["ERP158758"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0003789","EFO_0005518","EFO_0004184","EFO_0003969"]}}