biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsveronica manicardiNextSeq 500RNA-seq of total RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13925The aim of this experiment was to identify NEAT1-dependent transcriptional program in multiple myeloma. AMO-1 cell line was silenced for NEAT1 expression using antisense oligonucleotide gapmeR (g#N1_E). Not-targeting gapmeR was used as control (g#SCR). RNA was collected 96 hours after gapmeR delivery.biostudies-arrayexpressGrowth Protocol - AMO-1 were cultured in RPMI supplemented with 10% FBS and 1% Penicillin-Streptomycin.Nucleic Acid Extraction - Total RNA was extracted by TRIzol reagent according to the manufacturer’s instructions.Sequencing - Sequencing was performed by Illumina Nextseq 500 instrument (High output kit v2, 2x150 cycles).Sample Collection - Cells were collected after 96 hours from gymnotic delivery.Library Construction - RNAseq libraries were prepared by Illumina® Stranded Total RNA Prep kit starting from 500 ng of RNA. RNA quality was assessed by 4200 Tapestation using RNA screen tape kit protocol.Sample Treatment - Cells were seeded at 5x10^4 cells/ml and concurrently treated with naked gapmeR (g#N1_E and g#SCR).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB Filesveronica manicardifalseRNA-seq of AMO-1 multiple myeloma cell line silenced for lncRNA NEAT1 expression and relative control conditionThe aim of this experiment was to identify NEAT1-dependent transcriptional program in multiple myeloma. AMO-1 cell line was silenced for NEAT1 expression using antisense oligonucleotide gapmeR (g#N1_E). Not-targeting gapmeR was used as control (g#SCR). RNA was collected 96 hours after gapmeR delivery.2026-03-06T00:00:00Z2026-03-06T09:48:27.74Z2024-03-19T18:55:29.605ZE-MTAB-13925ERP158758EFO_0002944EFO_0004170EFO_0009653EFO_0003789EFO_0005518EFO_0004184EFO_0003969