{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Raya Faigenbaum-Romm"],"instrument_platform":["PacBio RS"],"study_type":["DNA-seq"],"organism":["mixed sample"],"species":["mixed sample"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13944"],"description":["Pacbio library preparation and sequencing was carried-out by BGI on one of the technical samples of the Microcolony-seq experiment (UTI_30).  A SMRTbell library was prepared and sequenced in a Pacbio Revio machine. The assembled genome was used for mapping the Microcolony-seq experiment"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Pacbio library preparation and sequencing was carried-out by BGI on one of the technical samples (UTI_30). SMRTbell prep kit 3.0 was used","Sequencing - Pacbio Revio machine","Nucleic Acid Extraction - DNA was extracted from 1 mL of bacteria using the DNeasy Blood & Tissue Kit (Qiagen).","Sample Collection - Microcolonies were selected from urine of a patient with a UTI infection on LB plate are kept at -80C. Bacteria were regrown in LB at 37C with shaking for 5h."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Raya Faigenbaum-Romm"],"additional_accession":[]},"is_claimable":false,"name":"Pacbio sequencing of bacteria from one colony from a urine of a patient with a urinary tract infection","description":"Pacbio library preparation and sequencing was carried-out by BGI on one of the technical samples of the Microcolony-seq experiment (UTI_30).  A SMRTbell library was prepared and sequenced in a Pacbio Revio machine. The assembled genome was used for mapping the Microcolony-seq experiment","dates":{"release":"2025-08-27T00:00:00Z","modification":"2025-08-28T00:02:26.036Z","creation":"2024-03-26T14:02:28.031Z"},"accession":"E-MTAB-13944","cross_references":{"ENA":["ERP158963"],"Biostudies":["E-MTAB-13941","E-MTAB-13892"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}