biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsRaya Faigenbaum-RommPacBio RSDNA-seqmixed samplemixed samplehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13944Pacbio library preparation and sequencing was carried-out by BGI on one of the technical samples of the Microcolony-seq experiment (UTI_30). A SMRTbell library was prepared and sequenced in a Pacbio Revio machine. The assembled genome was used for mapping the Microcolony-seq experimentbiostudies-arrayexpressLibrary Construction - Pacbio library preparation and sequencing was carried-out by BGI on one of the technical samples (UTI_30). SMRTbell prep kit 3.0 was usedSequencing - Pacbio Revio machineNucleic Acid Extraction - DNA was extracted from 1 mL of bacteria using the DNeasy Blood & Tissue Kit (Qiagen).Sample Collection - Microcolonies were selected from urine of a patient with a UTI infection on LB plate are kept at -80C. Bacteria were regrown in LB at 37C with shaking for 5h.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesRaya Faigenbaum-RommfalsePacbio sequencing of bacteria from one colony from a urine of a patient with a urinary tract infectionPacbio library preparation and sequencing was carried-out by BGI on one of the technical samples of the Microcolony-seq experiment (UTI_30). A SMRTbell library was prepared and sequenced in a Pacbio Revio machine. The assembled genome was used for mapping the Microcolony-seq experiment2025-08-27T00:00:00Z2025-08-28T00:02:26.036Z2024-03-26T14:02:28.031ZE-MTAB-13944ERP158963E-MTAB-13941E-MTAB-13892EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184