biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsBetty Ying-Wen ChungNextSeq 2000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14001QuantSeq of immortalised primary human foetal foreskin fibroblasts (HFFF-TERTs) infected with Sendai Virus (SeV) or mock infected. Experiments were performed in two rounds. In the first round cells were depleted of both CRTC2 and CRTC3 using siRNAs, or treated with a control siRNA. In the second round of experiments HFFF-TERT cells had either CRTC2, CRTC3 or both CRTC2&CRTC3 knocked out using a CRISPR-Cas9 system. Knock-out cells were complemented with either full length CRTC2, full length CRTC3, a version of CRTC2 lacking the N-terminal 50 amino acids (ΔN50), a version of CRTC3 lacking the N-terminal 50 amino acids (ΔN50), both full length CRTC2 and CRTC3, or a vector only control.biostudies-arrayexpressSample Collection - Cultured cells were treated as indicated and harvested 8 hours post infection.Sequencing - Libraries were sequenced by Cambridge Genomic Services on the Illumina NextSeq 2000 platformLibrary Construction - Libaries were prepared using a QuantSeq 3' mRNA-Seq Library Prep Kit-FWD from Lexogen.Nucleic Acid Extraction - Total RNA was harvested from cells at 8 hours post infection by acidic phenol-chloroform extraction.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesBetty Ying-Wen ChungfalseQuantSeq of HFFF-TERTs infected with Sendai VirusQuantSeq of immortalised primary human foetal foreskin fibroblasts (HFFF-TERTs) infected with Sendai Virus (SeV) or mock infected. Experiments were performed in two rounds. In the first round cells were depleted of both CRTC2 and CRTC3 using siRNAs, or treated with a control siRNA. In the second round of experiments HFFF-TERT cells had either CRTC2, CRTC3 or both CRTC2&CRTC3 knocked out using a CRISPR-Cas9 system. Knock-out cells were complemented with either full length CRTC2, full length CRTC3, a version of CRTC2 lacking the N-terminal 50 amino acids (ΔN50), a version of CRTC3 lacking the N-terminal 50 amino acids (ΔN50), both full length CRTC2 and CRTC3, or a vector only control.2026-04-10T00:00:00Z2026-04-10T01:02:15.746Z2024-04-12T10:17:18.087ZE-MTAB-14001ERP159375EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184