{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Fazio Grazia"],"instrument_platform":["NextSeq 550"],"study_type":["RNA-seq of total RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14034"],"description":["We performed transcriptome analysis to characterize II trimester of human Amniotic Fluid Extra Vesicles (hAF-EVs)"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - The libraries have been analysed by paired-end sequencing on NextSeq550 Illumina platform, 2x75.","Library Construction - Whole transcriptome RNA-seq analyses have been performed using the Universal RNAseq kit (Nugen, Tecan).","Sample Collection - II trimester human amniotic fluid (hAF) samples were collected as leftover material from prenatal screening. hAF samples were concentrated and processed by size exclusion chromatography (SEC). hAF-EV-enriched fractions were collected and concentrated by ultracentrifugation and then resuspended in a PBS solution.","Nucleic Acid Extraction - Combined phenol/guanidine-based lysis of samples and silicamembrane–based purification of total RNA. (miRNeasy Micro Kit, Qiagen)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Fazio Grazia"],"additional_accession":[]},"is_claimable":false,"name":"Whole Transcriptome Analysis (Rna-Seq) By Next-Generation Sequencing of hAF-EVs","description":"We performed transcriptome analysis to characterize II trimester of human Amniotic Fluid Extra Vesicles (hAF-EVs)","dates":{"release":"2025-05-01T00:00:00Z","modification":"2024-04-23T22:13:43.963Z","creation":"2024-04-23T22:13:43.963Z"},"accession":"E-MTAB-14034","cross_references":{"ENA":["ERP159831"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0004184"]}}