biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsFazio GraziaNextSeq 550RNA-seq of total RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14034We performed transcriptome analysis to characterize II trimester of human Amniotic Fluid Extra Vesicles (hAF-EVs)biostudies-arrayexpressSequencing - The libraries have been analysed by paired-end sequencing on NextSeq550 Illumina platform, 2x75.Library Construction - Whole transcriptome RNA-seq analyses have been performed using the Universal RNAseq kit (Nugen, Tecan).Sample Collection - II trimester human amniotic fluid (hAF) samples were collected as leftover material from prenatal screening. hAF samples were concentrated and processed by size exclusion chromatography (SEC). hAF-EV-enriched fractions were collected and concentrated by ultracentrifugation and then resuspended in a PBS solution.Nucleic Acid Extraction - Combined phenol/guanidine-based lysis of samples and silicamembrane–based purification of total RNA. (miRNeasy Micro Kit, Qiagen)OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesFazio GraziafalseWhole Transcriptome Analysis (Rna-Seq) By Next-Generation Sequencing of hAF-EVsWe performed transcriptome analysis to characterize II trimester of human Amniotic Fluid Extra Vesicles (hAF-EVs)2025-05-01T00:00:00Z2024-04-23T22:13:43.963Z2024-04-23T22:13:43.963ZE-MTAB-14034ERP159831EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184