biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsFazio GraziaNextSeq 2000RNA-seq of total RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14042We performed transcriptome analysis to investigate molecular effect of variants in HDAC2 and RSTS genes.biostudies-arrayexpressNucleic Acid Extraction - RNA was isolated using Trizol-chloroform-isopropyl alcohol extraction protocolLibrary Construction - Whole transcriptome RNA-seq analyses have been performed using the Universal RNAseq kit (Nugen, Tecan).Sequencing - The libraries have been analysed by paired-end sequencing on NextSeq2000 Illumina platform, 2x50.Sample Collection - Lymphoblastoid Cell Lines obtained from EBV-immortalized B-lymphocytes of patients'-donors' peripheral bloodOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesFazio GraziafalseWhole Transcriptome Analysis (Rna-Seq) By Next-Generation Sequencing of Rubinstein-Taybi syndrome (RSTS) Lymphoblastoid Cell Lines (LCLs)We performed transcriptome analysis to investigate molecular effect of variants in HDAC2 and RSTS genes.2025-04-30T00:00:00Z2024-05-01T08:55:08.604Z2024-05-01T08:55:08.604ZE-MTAB-14042ERP159973EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184