{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Trypanosoma brucei brucei"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14043"],"description":["Trypanosoma brucei is a kinetoplastid protozoan and the causal agent of Human African Trypanosomiasis. The life cycle is complex with multiple developmental forms. To identify gene regulations in different developmental forms, we carried out gene expression analysis of two replicative developmental forms which are readily recapitulated in laboratory culture: the ‘slender’ mammalian bloodstream form and the tsetse procyclic form."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - RNA library construction was carried out using an the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, with poly A selection of 1 microgram total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module, following the provided protocol.","Sample Collection - Cells were cultured in DTM medium with 3 mM citrate and 3 mM cis-aconitate at 27oC.","Sample Collection - Cells were cultured in HMI-11 medium for growth at 37 oC.","Nucleic Acid Extraction - Total RNA samples were prepared using the Qiagen RNAeasy Mini Kit.","Sequencing - Sequencing was performed using an Illumina NextSeq 500 or an MiSeq instrument."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"species":["Trypanosoma brucei brucei"],"additional_accession":["ERP159967"],"pubmed_authors":["Fumiko Kawasaki"]},"is_claimable":false,"name":"RNA sequencing data from Trypanosoma brucei brucei EATRO1125 before/after differentiation of bloodstream forms to procyclic forms","description":"Trypanosoma brucei is a kinetoplastid protozoan and the causal agent of Human African Trypanosomiasis. The life cycle is complex with multiple developmental forms. To identify gene regulations in different developmental forms, we carried out gene expression analysis of two replicative developmental forms which are readily recapitulated in laboratory culture: the ‘slender’ mammalian bloodstream form and the tsetse procyclic form.","dates":{"release":"2025-06-29T00:00:00Z","modification":"2025-03-26T05:28:59.547Z","creation":"2024-04-30T16:23:10.055Z"},"accession":"E-MTAB-14043","cross_references":{"ENA":["ERP159967"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}