biostudies-arrayexpressDrosophila melanogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14107Chromatin accessibility was assayed in control of salm/salr double knockdown third instar wing imaginal discs. Imaginal discs of the stated genotypes were fixed in PFA and treated for ATAC-seq. For each genotype, two biological replicates were collected and used to construct the ATAC libraries. Libraries were sequenced (paired-end) on Illumina NextSeq 500.biostudies-arrayexpressSequencing - paired-end sequencing on illumina NextSeq 500Library Construction - Disk were permeabilization: Thaw disks on ice for 10 min and add 50ul PBT+PI; Add 50 ul of PBT + 0.4% IGEPAL CA-630; Incubate at 4C for 30 min on a nutator and Spin at 3200 xg for 5 min at 4C. Tagmentation and reverse-crosslinking: Resuspend the nuclei pellet in 50 ul tagmentation mix(5 uL TDE1 (Illumina, 15027865), 25 uL 2x buffer (Illumina, 15027866), 20 uL nuclease-free H2O). Incubate at 37C for 30 min. Add 50 ul STOP buffer, 5 ul of 1mg/ml RNase A and incubate at 55C for 10 min. Add 3 ul Proteinase K (20 mg/ml) incubate 65C for 1 hour. Purify with Zymo DNA clean and concentrator kit. Finally samples are PCR amplified and size selected with SPRI beads.Growth Protocol - We used 20 imaginal wing discs per sample to make two technical replicates, and the larvae were of salEPV-Gal4 UAS-salm-i/+; UAS-salr-i/+ and salEPV-Gal4 UAS-GFP/+ genotypes. The discs were dissected in pre-cooled PBS, pipetted in PBT (PBS, 0.1 % Triton X-100) containing 1x proteases inhibitors (cOmplete™ Protease Inhibitor Cocktail tablets, Roche, 11873580001) and suspended in transposition solution containing.MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500ATAC-seqDrosophila melanogasterERP160391Jose F de CelisCharles GirardotfalseComparison of ATAC seq between control wing imaginal discs and salm/salr knockdown wing imaginal discsChromatin accessibility was assayed in control of salm/salr double knockdown third instar wing imaginal discs. Imaginal discs of the stated genotypes were fixed in PFA and treated for ATAC-seq. For each genotype, two biological replicates were collected and used to construct the ATAC libraries. Libraries were sequenced (paired-end) on Illumina NextSeq 500.2025-05-05T00:00:00Z2026-06-06T01:10:29.368Z2024-05-20T16:41:55.414ZE-MTAB-14107ERP160391EFO_0007045EFO_0004170EFO_0005518EFO_0004184