{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Arabidopsis thaliana"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14125"],"description":["Comparison analysis of Arabidopsis fbl17-1 single and fbl17-1 e2fc-1 double mutant transcriptomes to study the implication of FBL17 and E2FC in cell cycle regulation and DNA damage responses"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - mRNAs were isolated from total RNAs by using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB) for mRNA libraries preparation. Sequencing libraries were prepared using the Collibri stranded RNA library kit for Illumina (Invitrogen).","Growth Protocol - Arabidopsis sterile seeds are plated on MES-buffered Murashige and Skoog) medium (1X MS salts; Duchefa; 0.8% agar, Fluka; 1% (w/v) sucrose; pH adjusted to 5.7 with KOH). Plated seeds are stratified at 4°C for 2-3 days and then transferred to a plant growth chamber under a 16-h-light/8-h-dark cycle (22/20°C) until collection.","Nucleic Acid Extraction - Total RNAs were extracted from 10 day-old whole seedlings in vitro-grown by using Trizol solution (Invitrogen) completed by a second phenol/chloroform treatment. Three biological replicates were used as starting material. RNAs concentrations were determined with a QuBit Fluorometer (Thermo Fisher Scientific). RNAs integrities were checked using the 2100 Bioanalyzer (Agilent).","Sequencing - The libraries were sequenced using an Illumina Nextseq 500 system (single-end mode 1 × 75 bp).","Sample Collection - Whole 10 day-old in vitro-grown seedlings are snap-frozen in liquid nitrogen before RNA extraction."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"species":["Arabidopsis thaliana"],"additional_accession":["E-MTAB-9050"],"pubmed_authors":["Sandra Noir"]},"is_claimable":false,"name":"RNAseq analysis of Arabidopsis e2fc-1 and fbl17-1 e2fc-1 mutant seedlings","description":"Comparison analysis of Arabidopsis fbl17-1 single and fbl17-1 e2fc-1 double mutant transcriptomes to study the implication of FBL17 and E2FC in cell cycle regulation and DNA damage responses","dates":{"release":"2026-05-31T00:00:00Z","modification":"2026-05-31T01:01:14.409Z","creation":"2024-05-28T21:39:40.083Z"},"accession":"E-MTAB-14125","cross_references":{"ENA":["ERP160716"],"Biostudies":["E-MTAB-9050"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003738","EFO_0004184"]}}