biostudies-arrayexpressArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14125Comparison analysis of Arabidopsis fbl17-1 single and fbl17-1 e2fc-1 double mutant transcriptomes to study the implication of FBL17 and E2FC in cell cycle regulation and DNA damage responsesbiostudies-arrayexpressLibrary Construction - mRNAs were isolated from total RNAs by using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB) for mRNA libraries preparation. Sequencing libraries were prepared using the Collibri stranded RNA library kit for Illumina (Invitrogen).Growth Protocol - Arabidopsis sterile seeds are plated on MES-buffered Murashige and Skoog) medium (1X MS salts; Duchefa; 0.8% agar, Fluka; 1% (w/v) sucrose; pH adjusted to 5.7 with KOH). Plated seeds are stratified at 4°C for 2-3 days and then transferred to a plant growth chamber under a 16-h-light/8-h-dark cycle (22/20°C) until collection.Nucleic Acid Extraction - Total RNAs were extracted from 10 day-old whole seedlings in vitro-grown by using Trizol solution (Invitrogen) completed by a second phenol/chloroform treatment. Three biological replicates were used as starting material. RNAs concentrations were determined with a QuBit Fluorometer (Thermo Fisher Scientific). RNAs integrities were checked using the 2100 Bioanalyzer (Agilent).Sequencing - The libraries were sequenced using an Illumina Nextseq 500 system (single-end mode 1 × 75 bp).Sample Collection - Whole 10 day-old in vitro-grown seedlings are snap-frozen in liquid nitrogen before RNA extraction.MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNAArabidopsis thalianaE-MTAB-9050Sandra NoirfalseRNAseq analysis of Arabidopsis e2fc-1 and fbl17-1 e2fc-1 mutant seedlingsComparison analysis of Arabidopsis fbl17-1 single and fbl17-1 e2fc-1 double mutant transcriptomes to study the implication of FBL17 and E2FC in cell cycle regulation and DNA damage responses2026-05-31T00:00:00Z2026-05-31T01:01:14.409Z2024-05-28T21:39:40.083ZE-MTAB-14125ERP160716E-MTAB-9050EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184