{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Arabidopsis thaliana"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14134"],"description":["Col and h2a.w mutants (h2a.w6, h2a.w7, h2a.w6/7, h2a.w-2) bud sample were harvested. Nuclei extracted using Honda buffer was fragmented with mirococcal nuclease (MNase, NEB M0247S). The fragmented DNA was purified by phenol/chloroform/isoamyl alcohol (24:24:1) extraction, followed by ethanol precipitation and 2% agarose gel separation and gel extraction of ~145-150 bp DNA. Purified DNA  sent to BGI (Hong Kong) for library preparation and sequencing."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Phenol:Chloroform:Isoamyl alcohol(24:24:1) method","Sample Collection - buds of 6 week old plants","Library Construction - KAPA HyperPrep kit","Growth Protocol - long day conditions (16 hr light), 20 degrees Celcius","Sequencing - DNBseq G-400 PE100"],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["DNBSEQ-G400"],"study_type":["MNase-seq"],"species":["Arabidopsis thaliana"],"additional_accession":["ERP160755"],"pubmed_authors":["Kyuha Choi"]},"is_claimable":false,"name":"MNase-seq in Arabidopsis thaliana Col and h2a.w mutants","description":"Col and h2a.w mutants (h2a.w6, h2a.w7, h2a.w6/7, h2a.w-2) bud sample were harvested. Nuclei extracted using Honda buffer was fragmented with mirococcal nuclease (MNase, NEB M0247S). The fragmented DNA was purified by phenol/chloroform/isoamyl alcohol (24:24:1) extraction, followed by ethanol precipitation and 2% agarose gel separation and gel extraction of ~145-150 bp DNA. Purified DNA  sent to BGI (Hong Kong) for library preparation and sequencing.","dates":{"release":"2025-04-23T00:00:00Z","modification":"2026-06-05T22:35:30.26Z","creation":"2024-05-30T18:08:23.34Z"},"accession":"E-MTAB-14134","cross_references":{"ENA":["ERP160755"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0003751","EFO_0005518","EFO_0004184"]}}