{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Smiths Lueong"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14164"],"description":["Whole genome methylation sequencing was performed on multicancer pooles of patient plasma-derived DNA, buffy coat and healthy donnor plasma and buffy coat. Methylation values were extracted with Bismark methylation extractor"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - DNA was isolated from plasma and buffy coat using the Maxwell automated system","Library Construction - Methylation libraries were prepared using the enzymatic methyl-seq kit from New E´ngland Biolabs","Sample Collection - Blood samples were collected in EDTA tubes and plasma was prepared by double centrifugation. PBMCs well isolated using a Ficoll gradient","Sequencing - Libraries were sequenced 100 bp paired ends on an illumina Novaseq 6000 at the German Cancer Research Center","Sequencing - The sequencing was performed on an illumina NovaSeq 6000 with 100 base pairs paired end"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - No normalization was performed on Whole genome methylation sequencing data","Sequence Alignment - Raw reads were quality controled and trimmed with Trim galore and aligned to with Bismark"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["methylation profiling by high throughput sequencing"],"species":["Homo sapiens"],"pubmed_authors":["Smiths Lueong"],"additional_accession":[]},"is_claimable":false,"name":"Genome-scale methylation analysis uncovers epigenetic components of anti-tumor immunity and multicancer early detection markers","description":"Whole genome methylation sequencing was performed on multicancer pooles of patient plasma-derived DNA, buffy coat and healthy donnor plasma and buffy coat. Methylation values were extracted with Bismark methylation extractor","dates":{"release":"2025-06-30T00:00:00Z","modification":"2024-06-10T22:19:04.418Z","creation":"2024-06-10T22:19:04.418Z"},"accession":"E-MTAB-14164","cross_references":{"EFO":["EFO_0002944","EFO_0004170","EFO_0002761","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}