biostudies-arrayexpressSmiths LueongHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14164Whole genome methylation sequencing was performed on multicancer pooles of patient plasma-derived DNA, buffy coat and healthy donnor plasma and buffy coat. Methylation values were extracted with Bismark methylation extractorbiostudies-arrayexpressNucleic Acid Extraction - DNA was isolated from plasma and buffy coat using the Maxwell automated systemLibrary Construction - Methylation libraries were prepared using the enzymatic methyl-seq kit from New E´ngland BiolabsSample Collection - Blood samples were collected in EDTA tubes and plasma was prepared by double centrifugation. PBMCs well isolated using a Ficoll gradientSequencing - Libraries were sequenced 100 bp paired ends on an illumina Novaseq 6000 at the German Cancer Research CenterSequencing - The sequencing was performed on an illumina NovaSeq 6000 with 100 base pairs paired endOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - No normalization was performed on Whole genome methylation sequencing dataSequence Alignment - Raw reads were quality controled and trimmed with Trim galore and aligned to with BismarkUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000methylation profiling by high throughput sequencingHomo sapiensSmiths LueongfalseGenome-scale methylation analysis uncovers epigenetic components of anti-tumor immunity and multicancer early detection markersWhole genome methylation sequencing was performed on multicancer pooles of patient plasma-derived DNA, buffy coat and healthy donnor plasma and buffy coat. Methylation values were extracted with Bismark methylation extractor2025-06-30T00:00:00Z2024-06-10T22:19:04.418Z2024-06-10T22:19:04.418ZE-MTAB-14164EFO_0002944EFO_0004170EFO_0002761EFO_0004917EFO_0005518EFO_0003816EFO_0004184