biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsNaomi Asaharatranscription profiling by arrayMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14182Paraoxonase 1 (PON 1) binds to high-density lipoprotein (HDL) and is responsible for its anti-atherosclerotic effect through its antioxidant activity. This dataset contains gene expression data obtained from a study investigating the effects of human paraoxonase 1 treatment on acute ischemic stroke model mice. The study aimed to identify gene expression changes associated with the treatment and to understand the molecular mechanisms underlying the therapeutic effects of human paraoxonase 1 in acute ischemic stroke model mice. The dataset includes raw microarray data and associated metadata. The study was conducted using a middle cerebral artery (MCA) ischemia/reperfusion model in mice, and gene expression was measured in the brain tissue samples collected from control and treated mice. The dataset also includes information on the experimental design, sample collection, and data processing methods used in the study.biostudies-arrayexpressSample Collection - - The middle 2 mm slices (central 2 slices) containing the infarction site in the right cerebral hemisphere were collected from MCAI/R model mice treated with PON 1 or vehicle at 6, 24, and 72 hours after MCAI/R. -Control were collected immediately (0 hour) after MCAI/R from the MCAI/R model mice without administration.Hybridization - - Labeled cDNA was hybridized to the arrays at 45°C for 16 hours.Nucleic Acid Extraction - - RNA was prepared using ISOGEN, and RNA integrity and quantity were analyzed using photometry (NanoDrop, Thermo Fisher Scientific) and agarose gel electrophoresis. - The experiment was conducted with n=3 for each group and the prepared RNA samples were pooled in equal volumes for each group.Scaning - - Arrays were scanned using the Affymetrix GeneChip Scanner 3000. - Data were extracted using Affymetrix GeneChip Command Console Software (AGCC).Labeling - - 20µg of pooled total RNA was used for cDNA synthesis using the Affymetrix GeneChipTM 3'IVT Reagent Kit. - Fragmentation and labeling of cDNA were performed according to the Affymetrix　GeneChipTM Hybridization, Wash, and Stain Kit.Sample Treatment - - PON 1 or its solvent was administered intravenously once daily for three consecutive days. - The initial treatment was performed immediately after MCAI/R. - Controls were not dosed.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsNaomi AsaharaData Transformation - - Raw data (CEL files) were normalized using MAS5 (Affymetrix MicroArray Suite 5).falseMicroarray analysis of the brain in a mouse model of cerebral ischemia/reperfusion injury treated with human paraoxonase 1 against vehicle controlParaoxonase 1 (PON 1) binds to high-density lipoprotein (HDL) and is responsible for its anti-atherosclerotic effect through its antioxidant activity. This dataset contains gene expression data obtained from a study investigating the effects of human paraoxonase 1 treatment on acute ischemic stroke model mice. The study aimed to identify gene expression changes associated with the treatment and to understand the molecular mechanisms underlying the therapeutic effects of human paraoxonase 1 in acute ischemic stroke model mice. The dataset includes raw microarray data and associated metadata. The study was conducted using a middle cerebral artery (MCA) ischemia/reperfusion model in mice, and gene expression was measured in the brain tissue samples collected from control and treated mice. The dataset also includes information on the experimental design, sample collection, and data processing methods used in the study.2025-06-05T00:00:00Z2024-06-18T20:51:05.994Z2024-06-18T20:51:05.994ZE-MTAB-14182EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815EFO_0003969