{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Thomas MERCHER"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["ChIP-seq"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14187"],"description":["CBFA2T3::GLIS2 was fused with GFP through CRISPR-Cas9 in WSU-AML cells. Cells were then used for ChIP-seq using a GFP antibody in order to determined the CBFA2T3::GLIS2 fusion binding sites."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - For each replicate, 2M cells were fixed in 1% formaldehyde and reaction was stopped with 125mM Glycine.","Sequencing - Sequencings were performed using NovaSeq6000 (Illumina).","Library Construction - DNA fragments were amplified by PCR with indexed paired-end adaptors (Illumina).","Nucleic Acid Extraction - After cell lysis, chromatin was digested by Micrococcal nuclease(NEB) and sonicated during 15s with Bioruptor Plus (Diagenode) at medium intensity. Sheared chromatin was then used for immunoprecipitation."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Thomas MERCHER"],"additional_accession":[]},"is_claimable":false,"name":"ChIP-seq to identify binding sites of the fusion oncogene CBFA2T3::GLIS2 in WSU-AML cell line","description":"CBFA2T3::GLIS2 was fused with GFP through CRISPR-Cas9 in WSU-AML cells. Cells were then used for ChIP-seq using a GFP antibody in order to determined the CBFA2T3::GLIS2 fusion binding sites.","dates":{"release":"2025-07-09T00:00:00Z","modification":"2025-07-09T16:00:44.066Z","creation":"2024-06-19T15:26:22.006Z"},"accession":"E-MTAB-14187","cross_references":{"ENA":["ERP161297"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002692","EFO_0005518","EFO_0004184"]}}