biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsThomas MERCHERIllumina NovaSeq 6000ChIP-seqHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14187CBFA2T3::GLIS2 was fused with GFP through CRISPR-Cas9 in WSU-AML cells. Cells were then used for ChIP-seq using a GFP antibody in order to determined the CBFA2T3::GLIS2 fusion binding sites.biostudies-arrayexpressSample Collection - For each replicate, 2M cells were fixed in 1% formaldehyde and reaction was stopped with 125mM Glycine.Sequencing - Sequencings were performed using NovaSeq6000 (Illumina).Library Construction - DNA fragments were amplified by PCR with indexed paired-end adaptors (Illumina).Nucleic Acid Extraction - After cell lysis, chromatin was digested by Micrococcal nuclease(NEB) and sonicated during 15s with Bioruptor Plus (Diagenode) at medium intensity. Sheared chromatin was then used for immunoprecipitation.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesThomas MERCHERfalseChIP-seq to identify binding sites of the fusion oncogene CBFA2T3::GLIS2 in WSU-AML cell lineCBFA2T3::GLIS2 was fused with GFP through CRISPR-Cas9 in WSU-AML cells. Cells were then used for ChIP-seq using a GFP antibody in order to determined the CBFA2T3::GLIS2 fusion binding sites.2025-07-09T00:00:00Z2025-07-09T16:00:44.066Z2024-06-19T15:26:22.006ZE-MTAB-14187ERP161297EFO_0002944EFO_0004170EFO_0002692EFO_0005518EFO_0004184