{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Thomas MERCHER"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["ATAC-seq"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14190"],"description":["CD34+ cord blood cells were transduced with a control or DLX3-overexpressing lentivirus for 4 days. Then, cells were sorted an ATAC-seq was performed."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - CD34+ cord blood cells were transduced with lentivirus and sorted on GFP+ 4 days after. 5x104-1x105 GFP+ live-sorted cells were spun at 500g for 5 min, washed with cold DPBS, lysed in cold lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% NP40, 3min on ice) and immediately spun at 500g for 10 min. The pelleted nuclei were resuspended in 50 µL of transposase reaction mix (Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina) for 30 min at 39°C.","Library Construction - Librairies were constructed by PCR using the Nextera DNA CD index kit (Illumina) and the NEBNext Ultra II Q5 master mix (NEB).","Nucleic Acid Extraction - PCR purification was performed using Agencourt AMPure XP magnetic beads (Beckman Coulter A63880) in order to remove large fragments and remaining primers. Library quality was assessed using an Agilent 2100 Bioanalyzer using a High Sensitivity DNA chip (Agilent Technologies 5067-4626).","Sequencing - Libraries were sequenced using Novaseq-6000 sequencer (Illumina) (50bp paired-end reads)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Thomas MERCHER"],"additional_accession":[]},"is_claimable":false,"name":"Chromatin opening change in cord blood cells with or without overexpression of DLX3","description":"CD34+ cord blood cells were transduced with a control or DLX3-overexpressing lentivirus for 4 days. Then, cells were sorted an ATAC-seq was performed.","dates":{"release":"2025-07-09T00:00:00Z","modification":"2025-07-09T16:01:10.011Z","creation":"2024-06-20T16:14:16.151Z"},"accession":"E-MTAB-14190","cross_references":{"ENA":["ERP161330"],"Biostudies":["E-MTAB-14188","E-MTAB-14189"],"EFO":["EFO_0002944","EFO_0007045","EFO_0004170","EFO_0005518","EFO_0004184"]}}