biostudies-arrayexpressPetr NazarovMus musculusTranscriptome Analysis Console (TAC v.4.0.1.36)https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14199In this study, we investigated the effects of metformin on the differentiation of bone marrow-derived macrophages (BMDMs) and their potential role in cancer immunity. BMDMs were generated from bone marrow cells extracted from C57BL/6N mice and differentiated in the presence or absence of metformin. Our transcriptomic analysis, performed using the Clariom S Mouse microarrays, revealed changes in gene expression profiles between metformin-treated and untreated macrophages.biostudies-arrayexpressGrowth Protocol - Bone marrow-derived macrophages (BMDMs) were differentiated as previously described. Briefly, C57BL/6N mice were euthanized via cervical dislocation and legs dissected out. Tibias and femurs bone marrow were collected through flushing. Red blood cells (RBCs) were lysed and bone marrow cells were cultured in low-attachment conditions for 7 days in DMEM GlutaMax (Gibco/Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Gibco/Life Technologies), 1% HEPES (Life Technologies), 100 U/mL pen-strep (Lonza), and 20 ng/mL of m-CSF (R&D Systems), with or without metformin hydrochloride (Merck). An additional complete medium was added on day 4.Sample Treatment - Bone marrow cells were cultured in low-attachment conditions for 7 days in DMEM GlutaMax (Gibco/Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Gibco/Life Technologies), 1% HEPES (Life technologies), 100 U/mL pen-strep (Lonza), and 20 ng/mL of m-CSF (R&D Systems), with or without 2 mM metformin hydrochloride (Merck).Nucleic Acid Extraction - RNA was extracted using the RNeasy kit (Qiagen) according the manufacturer’ instructions. Total RNA quantification was performed using the Nanodrop (Thermo Scientific) and its integrity was analyzed using the Qubit fluorometer (Invitrogen). Gene-level expression microarray-based analysis has been performed throughout the mouse Clariom S Pico assay (Applied Biosystems) according to the manufacturer’s instructions.Labeling - Thermo Ficher Clariom™ S (mouse) arrays were prepared in accordance with the standard protocol.Scaning - Thermo Ficher Clariom™ S (mouse) arrays were prepared in accordance with the standard protocol.Sample Collection - Fully differentiated bone marrow-derived macrophages were harvestedHybridization - Thermo Ficher Clariom™ S (mouse) arrays were prepared in accordance with the standard protocol.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - Microarray data were normalized and summarized using the SST-RMA algorithm in the Transcriptome Analysis Console (TAC v.4.0.1.36). Only mRNAs that show log2 expression above 7 in at least one sample were considered for further analysis in R/Bioconductor v.4.1.0.UnknownTranscriptomicsGenomicsProteomicsQiagenClariom S Mousetranscription profiling by arrayMus musculusNathalie NicotAndrea ScafidiPetr NazarovAlessandro MicheluccifalseMicroarray analysis of macrophages derived from mouse bone marrow cells with or without metformin treatmentIn this study, we investigated the effects of metformin on the differentiation of bone marrow-derived macrophages (BMDMs) and their potential role in cancer immunity. BMDMs were generated from bone marrow cells extracted from C57BL/6N mice and differentiated in the presence or absence of metformin. Our transcriptomic analysis, performed using the Clariom S Mouse microarrays, revealed changes in gene expression profiles between metformin-treated and untreated macrophages.2025-06-13T00:00:00Z2024-06-20T19:30:01.752Z2024-06-20T19:30:01.752ZE-MTAB-14199EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0003789EFO_0005518EFO_0003816EFO_0003815EFO_0003969