biostudies-arrayexpressChristina FissounMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14217When telomeres become excessively short, the cell enters senescence. At this point, it produces a protein called p21 in a significant manner. Only a protein called telomerase is capable of lengthening telomeres, but it is not present in adult cells. The aim of this project is to assess and study the benefit provided by telomerase in the context of osteoarthritis (using the CiOA preclinical model). We will utilize heterozygous transgenic mice: p21/mTERT, which possess a preserved copy of p21, along with the transgene. Therefore, we will need to compare our mice with control mice: p21+/+ that do not express the transgene. For each genotype, we will induce osteoarthritis pathology (CiOA) and compare it with the Sham group. In addition, we investigated whether ectopic expression of telomerase could protect aged mice against age-related osteoarthritis. Each group is in triplicate.biostudies-arrayexpressSample Collection - Harvested knees joint were dissected using surgical scissors, snap frozen immediately, and then stored in liquid nitrogen until the extraction step.Nucleic Acid Extraction - The grinding of the whole joint was performed using a pestle and mortar in liquid nitrogen. Total RNA from the resulting powder tissue was extracted using TRI Reagent (Sigma Aldrich #T94424) and RNeasy Kit (QIAGEN) according to the manufacturer’s instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop ND-1000 spectrophotometer and the Agilent 2100.Sequencing - Nucleic acid sequencing protocol was performed according to the manufacturer’s instructions by the Beijing Genomic Institute (BGI).Library Construction - Nucleic acid library construction protocol was performed according to the manufacturer’s instructions by the Beijing Genomic Institute (BGI).Sample Treatment - Protocol for young mice: The induction of osteoarthritis will be performed by injecting 5 μl of type VII collagenase (1U/5μl) into the joint of one of the mouse's knees on day 0 and day 2 (the other knee being injected with physiological saline solution). The mice will be anesthetized with isoflurane, and the leg will be shaved at the knee joint. A small incision (2mm) of the skin will be made at the central part of the joint. The needle of a 10μl Hamilton syringe will be inserted at the center of the patellar tendon to reach the intra-articular space, and the collagenase solution (or the physiological saline solution for the Sham knee joint) will be injected. The wound is sterilized with betadine. CiOA mice were euthanized at day 42 after the first collagenase injection. Protocol for old mice: Osteoarthritis in older mice is spontaneous age-related. Therefore, the mice underwent no interventions. They were simply euthanized at 18 months.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - We used Bowtie2 to map the clean reads to the reference gene sequence (transcriptome), and then used RSEM to calculate the gene expression level of each sample. Differential Gene Expression was performed using DESeq2.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsDNBSEQ-T7DNBSEQ Eukaryotic Stranded Transcriptome library preparation- Stranded mRNA LibraryRNA-seq of coding RNAMus musculusJean-Marc BrondelloChristina FissounfalseRNA-seq from whole osteoarthritic murine knee joint induced by CiOA model and spontaneous age-related model in p21/mTERT transgenic mice and their littermate controlsWhen telomeres become excessively short, the cell enters senescence. At this point, it produces a protein called p21 in a significant manner. Only a protein called telomerase is capable of lengthening telomeres, but it is not present in adult cells. The aim of this project is to assess and study the benefit provided by telomerase in the context of osteoarthritis (using the CiOA preclinical model). We will utilize heterozygous transgenic mice: p21/mTERT, which possess a preserved copy of p21, along with the transgene. Therefore, we will need to compare our mice with control mice: p21+/+ that do not express the transgene. For each genotype, we will induce osteoarthritis pathology (CiOA) and compare it with the Sham group. In addition, we investigated whether ectopic expression of telomerase could protect aged mice against age-related osteoarthritis. Each group is in triplicate.2025-12-31T00:00:00Z2025-12-31T02:02:14.063Z2024-07-03T15:08:09.051ZE-MTAB-14217ERP161616EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969