{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Dayeon Kang"],"instrument_platform":["Illumina NovaSeq 6000","-"],"study_type":["RNA-seq of coding RNA"],"organism":["Canis lupus familiaris"],"species":["Canis lupus familiaris"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14232"],"description":["Sexual dimorphism in dogs (Canis lupus familiaris) manifests through pronounced differences in morphology, physiology, and disease susceptibility. Despite early neutering, sex-specific differences in dogs persist, highlighting the need to investigate factors beyond sex hormones that contribute to these characteristics. This study investigated sex-specific epigenetic modifications in neutered dogs. The results indicated hormone-independent, sex-related differentially methylated genes related to oncogenic signaling and neuronal pathways. The differences in methylation status between the sexes were significantly associated with alterations in gene expression, indicating that methylation plays a regulatory role in gene transcription. Identification of canine XIST, previously annotated as LOC102156855, suggested a conserved mechanism of X-chromosome inactivation across species and a sex-specific epigenetic imprint on the genome, which is maintained independent of sex hormones. These findings enrich the understanding of sex-specific biology in dogs and highlight the intricate interplay between epigenetic modification and gene expression in determining sex-specific phenotypes and disease susceptibilities."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Libraries were prepared for 150 bp paired-end sequencing using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).","Sequencing - paired-end sequencing (2×150 bp) was performed using Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA).","Sample Collection - Eight beagle dogs (four males and four females) were investigated for sexual dimorphism in their transcriptomes. All dogs were five-year-old and had been neutered (castrated or spayed) before one year of age, and their healthy physical conditions were maintained through continuous monitoring, vaccination, and anthelmintic treatment. The experiment was ethically approved by the Institutional Animal Care and Use Committee of National Institute of Animal Science (NIAS 2022-586).","Nucleic Acid Extraction - Whole blood samples were collected using a 21-gauge needle and 5-mL syringe from the jugular vein of each dog. RNA from whole blood was extracted using a PAXgene Blood RNA Kit (762174; QIAGEN, Germantown, MD, USA) following the manufacturer’s instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Dayeon Kang"],"additional_accession":[]},"is_claimable":false,"name":"Epigenetic landscape of hormone-independent sexual dimorphism and characterization of canine XIST","description":"Sexual dimorphism in dogs (Canis lupus familiaris) manifests through pronounced differences in morphology, physiology, and disease susceptibility. Despite early neutering, sex-specific differences in dogs persist, highlighting the need to investigate factors beyond sex hormones that contribute to these characteristics. This study investigated sex-specific epigenetic modifications in neutered dogs. The results indicated hormone-independent, sex-related differentially methylated genes related to oncogenic signaling and neuronal pathways. The differences in methylation status between the sexes were significantly associated with alterations in gene expression, indicating that methylation plays a regulatory role in gene transcription. Identification of canine XIST, previously annotated as LOC102156855, suggested a conserved mechanism of X-chromosome inactivation across species and a sex-specific epigenetic imprint on the genome, which is maintained independent of sex hormones. These findings enrich the understanding of sex-specific biology in dogs and highlight the intricate interplay between epigenetic modification and gene expression in determining sex-specific phenotypes and disease susceptibilities.","dates":{"release":"2025-07-02T00:00:00Z","modification":"2024-07-08T16:45:43.273Z","creation":"2024-07-08T16:45:43.273Z"},"accession":"E-MTAB-14232","cross_references":{"ENA":["ERP161838"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}