{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Xiaodong Zhao"],"instrument_platform":["Illumina HiSeq 2000"],"study_type":["RIP-seq"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14256"],"description":["YTHDF2 displays extensive-expression patterns during oocyte maturation and its deficiency causes female infertility in mice. However, its specific mechanism of regulation remains elusive due to the absence of suitable in vitro models. FGSCs possess the capacity for self-renewal and differentiation into oocytes to support reproduction. The successful establishment of a line of FGSCs provides a platform for scientific research on female fertility and oogenesis. To understand how YTHDF2 exerts its regulatory effects on FGSCs, we conducted MeRIP-seq assay in FGSCs and aimed to identified YTHDF2 target transcripts."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - RNA was purified by NEBNext® rRNA Depletion Kit (NEB, USA). Fragmented and immunoprecipitated with anti-m6A antibody (Synaptic Systems, 202003). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).","Nucleic Acid Extraction - Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer’s protocols.","Sample Collection - FGSCs were cultured on STO feeder cells using Minimum Essential Medium-ɑ, supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 1 mM non-essential amino acid , 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 10 ng/mL mouse epidermal growth factor , 10 ng/mL mouse leukemia inhibitory factor, 40 ng/mL mouse glial cell line-derived neurotrophic factor, 10 ng/mL human basic fibroblast growth factor, and 1% penicillin-streptomycin. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected.","Sequencing - All libraries were sequenced at 150 bp paired-end on HiSeq 2000 (Illumina, USA)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Xiaodong Zhao"],"additional_accession":[]},"is_claimable":false,"name":"MeRIP sequencing analysis of female germline stem cells (FGSCs)","description":"YTHDF2 displays extensive-expression patterns during oocyte maturation and its deficiency causes female infertility in mice. However, its specific mechanism of regulation remains elusive due to the absence of suitable in vitro models. FGSCs possess the capacity for self-renewal and differentiation into oocytes to support reproduction. The successful establishment of a line of FGSCs provides a platform for scientific research on female fertility and oogenesis. To understand how YTHDF2 exerts its regulatory effects on FGSCs, we conducted MeRIP-seq assay in FGSCs and aimed to identified YTHDF2 target transcripts.","dates":{"release":"2025-07-26T00:00:00Z","modification":"2024-07-19T15:19:37.515Z","creation":"2024-07-19T15:19:37.515Z"},"accession":"E-MTAB-14256","cross_references":{"ENA":["ERP162164"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005310","EFO_0005518","EFO_0004184"]}}