biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsXiaodong ZhaoIllumina HiSeq 2000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14257YTHDF2 displays extensive-expression patterns during oocyte maturation and its deficiency causes female infertility in mice. However, its specific mechanism of regulation remains elusive due to the absence of suitable in vitro models. Female germline stem cells (FGSCs) possess the capacity for self-renewal and differentiation into oocytes to support reproduction. The successful establishment of a line of FGSCs provides a platform for scientific research on female fertility and oogenesis. To understand how YTHDF2 exerts its regulatory effects on FGSCs, we conducted RNA-seq assay in WT and Ythdf2-KO FGSCs and aimed to identified YTHDF2-responsive genes.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer’s protocols.Library Construction - mRNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Beads (NEB, USA). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).Sample Collection - FGSCs were cultured on STO feeder cells using Minimum Essential Medium-ɑ, supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 1 mM non-essential amino acid , 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 10 ng/mL mouse epidermal growth factor , 10 ng/mL mouse leukemia inhibitory factor, 40 ng/mL mouse glial cell line-derived neurotrophic factor, 10 ng/mL human basic fibroblast growth factor, and 1% penicillin-streptomycin. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected.Sequencing - All libraries were sequenced at 150 bp paired-end on HiSeq 2000 (Illumina, USA).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesXiaodong ZhaofalseRNA sequencing analysis of WT and Ythdf2-KO FGSCsYTHDF2 displays extensive-expression patterns during oocyte maturation and its deficiency causes female infertility in mice. However, its specific mechanism of regulation remains elusive due to the absence of suitable in vitro models. Female germline stem cells (FGSCs) possess the capacity for self-renewal and differentiation into oocytes to support reproduction. The successful establishment of a line of FGSCs provides a platform for scientific research on female fertility and oogenesis. To understand how YTHDF2 exerts its regulatory effects on FGSCs, we conducted RNA-seq assay in WT and Ythdf2-KO FGSCs and aimed to identified YTHDF2-responsive genes.2025-07-26T00:00:00Z2024-07-19T15:18:30.634Z2024-07-19T15:18:30.634ZE-MTAB-14257ERP162165EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184