{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Christophe Lambing"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Arabidopsis thaliana"],"species":["Arabidopsis thaliana"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14268"],"description":["Floral buds at meiotic stage from Col, h2a.w7, cmt3 and h2a.w7 cmt3 Arabidopsis thaliana plants were extracted as previously described (Lambing at al, 2020 Plant Cell - PMID: 32024691), and cDNA used to produce libraries."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - 100 mg of unopened flower buds was ground in liquid nitrogen and RNA was extracted using TRIzol reagent.","Library Construction - Six micrograms of total RNA per sample was treated with DNase I. Four micrograms of total DNase I–treated total RNA was then treated with Ribo-Zero rRNA Removal Kit (MRZPL116, Epicentre) to deplete rRNA from the RNA sample. Fifty nanograms of rRNA-depleted DNase I–treated RNA was used to prepare RNA-seq libraries using the ScriptSeq v2 RNA-seq Library Preparation Kit (SSV21124, Epicentre)","Growth Protocol - Plants were grown under long day conditions (16 hours light/8 hours dark) at 20°C.","Sequencing - RNA-seq libraries were sequenced on an Illumina NextSeq 500 instrument to generate paired-end (2×76 bp) reads.","Sample Collection - Unopened flower buds at floral stage 9 were collected in tubes, snap frozen in liquid nitrogen and stored at -80°C until further use."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Christophe Lambing"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of Col, h2a.w7, cmt3 and h2a.w7 cmt3 Arabidopsis thaliana floral buds","description":"Floral buds at meiotic stage from Col, h2a.w7, cmt3 and h2a.w7 cmt3 Arabidopsis thaliana plants were extracted as previously described (Lambing at al, 2020 Plant Cell - PMID: 32024691), and cDNA used to produce libraries.","dates":{"release":"2025-07-13T00:00:00Z","modification":"2024-07-22T10:16:40.461Z","creation":"2024-07-22T10:16:40.461Z"},"accession":"E-MTAB-14268","cross_references":{"ENA":["ERP162183"],"Biostudies":["E-MTAB-7371"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003738","EFO_0004184"]}}