biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsChristophe LambingNextSeq 500RNA-seq of coding RNAArabidopsis thalianaArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14268Floral buds at meiotic stage from Col, h2a.w7, cmt3 and h2a.w7 cmt3 Arabidopsis thaliana plants were extracted as previously described (Lambing at al, 2020 Plant Cell - PMID: 32024691), and cDNA used to produce libraries.biostudies-arrayexpressNucleic Acid Extraction - 100 mg of unopened flower buds was ground in liquid nitrogen and RNA was extracted using TRIzol reagent.Library Construction - Six micrograms of total RNA per sample was treated with DNase I. Four micrograms of total DNase I–treated total RNA was then treated with Ribo-Zero rRNA Removal Kit (MRZPL116, Epicentre) to deplete rRNA from the RNA sample. Fifty nanograms of rRNA-depleted DNase I–treated RNA was used to prepare RNA-seq libraries using the ScriptSeq v2 RNA-seq Library Preparation Kit (SSV21124, Epicentre)Growth Protocol - Plants were grown under long day conditions (16 hours light/8 hours dark) at 20°C.Sequencing - RNA-seq libraries were sequenced on an Illumina NextSeq 500 instrument to generate paired-end (2×76 bp) reads.Sample Collection - Unopened flower buds at floral stage 9 were collected in tubes, snap frozen in liquid nitrogen and stored at -80°C until further use.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesChristophe LambingfalseRNA-seq of Col, h2a.w7, cmt3 and h2a.w7 cmt3 Arabidopsis thaliana floral budsFloral buds at meiotic stage from Col, h2a.w7, cmt3 and h2a.w7 cmt3 Arabidopsis thaliana plants were extracted as previously described (Lambing at al, 2020 Plant Cell - PMID: 32024691), and cDNA used to produce libraries.2025-07-13T00:00:00Z2024-07-22T10:16:40.461Z2024-07-22T10:16:40.461ZE-MTAB-14268ERP162183E-MTAB-7371EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184