{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Madeleine Birgersson"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14294"],"description":["Granulosa cell tumors (GCTs) are rare types of ovarian cancer, consisting of an adult and a juvenile subtype. There has been a lack of research into this specific cancer, especially regarding the differences between the two subtypes. Here we compared the transcriptome of normal ovary (n=1) and GCT (n=5), and characterized the differences between the adult (n=3) and juvenile (n=2) subtype."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - The library was constructred using Illumina TruSeq Stranded Total RNA, including rRNA depletion with Illumina RiboZero.","Sample Collection - Samples were collected from patients undergoing surgery. Granulosa cell tumors (3 adult and 2 juvenile) and one matched normal ovarynwere snap frozen and stored at -70C.","Nucleic Acid Extraction - The samples were homogenized using a tissue lyser. Total RNA was then extracted using TRIzol and purified with the miRNeasy Mini Kit (Qiagen). On-column DNAse digestion was used to remove residual amounts of genomic DNA.","Sequencing - Samples were sequenced on the Illumina NovaSeq 6000 platform with S2 flow cell and 2x51 setup. The Bcl to FastQ conversion was performed using bcl2fastq_v2.19.1.403 from the CASAVA software suite."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - All reads were mapped against the human genome (GRCh37) using STAR. FeatureCounts was used to generate the gene counts for all samples."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Madeleine Birgersson"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of the granulosa cell tumor transcriptome using RNA-seq","description":"Granulosa cell tumors (GCTs) are rare types of ovarian cancer, consisting of an adult and a juvenile subtype. There has been a lack of research into this specific cancer, especially regarding the differences between the two subtypes. Here we compared the transcriptome of normal ovary (n=1) and GCT (n=5), and characterized the differences between the adult (n=3) and juvenile (n=2) subtype.","dates":{"release":"2026-05-28T00:00:00Z","modification":"2026-05-28T01:01:18.143Z","creation":"2024-07-24T22:32:36.758Z"},"accession":"E-MTAB-14294","cross_references":{"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}