biostudies-arrayexpressMadeleine BirgerssonHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14294Granulosa cell tumors (GCTs) are rare types of ovarian cancer, consisting of an adult and a juvenile subtype. There has been a lack of research into this specific cancer, especially regarding the differences between the two subtypes. Here we compared the transcriptome of normal ovary (n=1) and GCT (n=5), and characterized the differences between the adult (n=3) and juvenile (n=2) subtype.biostudies-arrayexpressLibrary Construction - The library was constructred using Illumina TruSeq Stranded Total RNA, including rRNA depletion with Illumina RiboZero.Sample Collection - Samples were collected from patients undergoing surgery. Granulosa cell tumors (3 adult and 2 juvenile) and one matched normal ovarynwere snap frozen and stored at -70C.Nucleic Acid Extraction - The samples were homogenized using a tissue lyser. Total RNA was then extracted using TRIzol and purified with the miRNeasy Mini Kit (Qiagen). On-column DNAse digestion was used to remove residual amounts of genomic DNA.Sequencing - Samples were sequenced on the Illumina NovaSeq 6000 platform with S2 flow cell and 2x51 setup. The Bcl to FastQ conversion was performed using bcl2fastq_v2.19.1.403 from the CASAVA software suite.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - All reads were mapped against the human genome (GRCh37) using STAR. FeatureCounts was used to generate the gene counts for all samples.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensMadeleine BirgerssonfalseCharacterization of the granulosa cell tumor transcriptome using RNA-seqGranulosa cell tumors (GCTs) are rare types of ovarian cancer, consisting of an adult and a juvenile subtype. There has been a lack of research into this specific cancer, especially regarding the differences between the two subtypes. Here we compared the transcriptome of normal ovary (n=1) and GCT (n=5), and characterized the differences between the adult (n=3) and juvenile (n=2) subtype.2026-05-28T00:00:00Z2026-05-28T01:01:18.143Z2024-07-24T22:32:36.758ZE-MTAB-14294EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184