biostudies-arrayexpressMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14299Analysis of global variability of translation between the Eif6 WT control genotype (CTRL) and the Eif6 S235A (SA) mutated MEF lines. The analysis is done on three replicates per genotype. Ribosome Protected Fragments (RPFs) were obtained following the procedure reported in McGlincy NJ & Ingolia NT. Methods. 2017 (doi: 10.1016/j.ymeth.2017.05.028).biostudies-arrayexpressSample Collection - Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cyclohexamide.Library Construction - Immagina Biotechnology LACEseq #LS-001_12Nucleic Acid Extraction - Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cyclohexamide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028).Sequencing - 500 pM library, Phix 10%, NextSeq 1000/2000 P2 100 cycles Illumina Kit, sequenced with NextSeq 2000MINSEQE ScoreAssays and DataProcessed DataorganisationMAGE-TAB FilesData Transformation - The raw fastq files underwent trimming and UMI extraction as reported in the Laceseq kit guidelines. Reads aligning to tRNAs and ncRNAs were removed using bowtie2.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000Ribo-seqMus musculusERP162738Ivan FerrarifalseRibo-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)Analysis of global variability of translation between the Eif6 WT control genotype (CTRL) and the Eif6 S235A (SA) mutated MEF lines. The analysis is done on three replicates per genotype. Ribosome Protected Fragments (RPFs) were obtained following the procedure reported in McGlincy NJ & Ingolia NT. Methods. 2017 (doi: 10.1016/j.ymeth.2017.05.028).2026-02-10T00:00:00Z2026-05-26T22:02:01.725Z2024-07-25T15:21:20.162ZE-MTAB-14299ERP162738EFO_0002944EFO_0004170EFO_0008891EFO_0005518EFO_0003816EFO_0004184