biostudies-arrayexpressRichard Barrett-JolleyRattus rattushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14300Study comparing transcriptome of different rat tissues at two age points. No There were no interventions. Same animals used for each tissue.biostudies-arrayexpressNucleic Acid Extraction - RNA extraction was performed using the RNeasy Fibrous Tissues kit (Qiagen, UK) as per manufacturer’s instructions. Approx. 30mg of tissue was lysed and homogenised using an ultra turrax (IKA Homogeniser, Sigma, UK) in buffer RLT before proteinase K digestion steps, total RNA bound followed by several washing steps and DNase digestion and total RNA eluted. Total RNA integrity was confirmed using the Bioanalyzer (Agilent Technologies, USA). Ribosomal RNA (rRNA) was depleted from RNA samples by using the Ribo-Zero™ rRNA Removal Kit (Epicentre, USA) in accordance with the instructions of the manufacturer.Growth Protocol - Naturally Ageing AgingSample Treatment - Natural Ageing AgingLibrary Construction - NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina, so they are paired-end reads but the orientation of the RNA strand is preserved in the first strandSample Collection - Rats were euthanised by a Schedule one procedure and the cardiac left ventricle dissected and snap frozen in RNALater.Sequencing - Illumina Hiseq 4000 and NovaSeq series sequencer 2x150OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - raw FASTQData Transformation - These are raw data with adapters removed.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNARattus rattusRichard Barrett-JolleyfalseRat AgeStudy comparing transcriptome of different rat tissues at two age points. No There were no interventions. Same animals used for each tissue.2025-09-01T00:00:00Z2025-09-01T01:02:25.978Z2024-07-26T13:37:08.805ZE-MTAB-14300ERP162775EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969