biostudies-arrayexpressMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14304Analysis of the global variability of gene expression between the Eif6 WT control genotype (CTRL) and the Eif6 S235A mutated (SA) MEF lines. To analyze the effects over time of the lack of Eif6 phosphorylation, we transduced MEFs with tamoxifen-inducible Cre, so that treatment leads to excision of wild-type exons and the generation of cells bearing the S235A mutation. The analysis is done on three replicates per genotype.biostudies-arrayexpressSample Collection - Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cyclohexamide.Library Construction - Novogene WTSNucleic Acid Extraction - RNA was extraced using TRIzol™ Reagent (Invitrogen) following manufacturer's instructionsSequencing - Novogene WTS paired-end 150MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of total RNAMus musculusERP162814Ivan FerrarifalseRNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)Analysis of the global variability of gene expression between the Eif6 WT control genotype (CTRL) and the Eif6 S235A mutated (SA) MEF lines. To analyze the effects over time of the lack of Eif6 phosphorylation, we transduced MEFs with tamoxifen-inducible Cre, so that treatment leads to excision of wild-type exons and the generation of cells bearing the S235A mutation. The analysis is done on three replicates per genotype.2026-02-10T00:00:00Z2026-02-10T09:32:30.532Z2024-07-29T21:37:39.288ZE-MTAB-14304ERP162814EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184