biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsBen TowlerNextSeq 500Ribo-seqDrosophila melanogasterDrosophila melanogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14344Ribo-seq and polyA-RNA seq of Control, pacman (XRN1) mutant and dis3L2 mutant L3 larvae. snoRNA-seq (75-150nt) from control and pacman mutant wing imaginal discs.biostudies-arrayexpressLibrary Construction - Ribo-seq and RNA-seq performed using NEB NEXT small RNA kit. snoRNA-seq performed using Illumina TruSeq small RNA kit.Sequencing - Sequencing performed on a NextSeq 500Nucleic Acid Extraction - RNA extracted by phenol chloroform and ethanol precipitationSample Collection - 120r male wandering L3 larvae of each genotype were collected, dissected and snap frozen in liquid nitrogen and stored at -80 until required.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesBen TowlerfalseThe 5’-3’ exoribonuclease Pacman is involved in long-non-coding RNA degradation and non-canonical translation eventsRibo-seq and polyA-RNA seq of Control, pacman (XRN1) mutant and dis3L2 mutant L3 larvae. snoRNA-seq (75-150nt) from control and pacman mutant wing imaginal discs.2026-08-09T00:00:00Z2026-08-09T01:00:47.339Z2024-08-09T09:45:43.129ZE-MTAB-14344ERP163123EFO_0002944EFO_0004170EFO_0008891EFO_0005518EFO_0004184