biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMarina ChekulaevaNextSeq 500RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14354induced motor neurons (iMNs) differentiated from hiPSC line were separated on axonal and somatodendritic compartments. Isolated subcellualr compartments were subjected to RNA-seq. FUSR244RR-ALS model was compared with isogenic control in which R244RR mutation was corrected to WT.biostudies-arrayexpressSequencing - Libraries were sequenced on an Illumina NextSeq 500 sequencer with single-end 150 bp reads.Nucleic Acid Extraction - RNA was extracted from using TRIfast (PeqLab 30-2010). For a detailed description of harvesting material from filter membranes see our prior work (Ludwik et al., 2019, doi: 10.1016/j.ymeth.2019.02.002).Library Construction - 100 ng of total RNA obtained from either total cells or separated compartments was used for library preparation with the Truseq stranded mRNA library prep kit (Illumina 20020595) according to the manufacturer’s recommendation.Sample Collection - Pateint-derived iPSC with FUS(R244RR) and isogenic line with NGN2, ISL1, and LHX3 cassettes were differentiated into induced motor neurons (iMNs) using established protocols (Fernandopulle et al., 2018, doi: 10.1002/cpcb.51). Cells were grown on filter membrane inserts (pore size 1.0 μm PET membrane for 6-well plates Millicell PLRP06H48, Millipore) to isolate axonal and somatodendritic compartments. Whole iMNs and subcellular compartments were collected on day 21 of the differentiation protocol.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMarina ChekulaevafalseRNA-seq of subcellular neuronal compartments of ALS modelinduced motor neurons (iMNs) differentiated from hiPSC line were separated on axonal and somatodendritic compartments. Isolated subcellualr compartments were subjected to RNA-seq. FUSR244RR-ALS model was compared with isogenic control in which R244RR mutation was corrected to WT.2025-08-05T00:00:00Z2024-08-12T20:51:15.55Z2024-08-12T20:51:15.55ZE-MTAB-14354ERP163205EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184