biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMarina ChekulaevaNextSeq 500RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14359induced motor neurons (iMNs) were differentiated from hiPSC line and different timepoints (day 1, 2, 4, 7, 14, 21) were subjected to RNA-seqbiostudies-arrayexpressSequencing - Libraries were sequenced on an Illumina NextSeq 500 sequencer with single-end 150 bp reads.Nucleic Acid Extraction - RNA was extracted from using TRIfast (PeqLab 30-2010). For a detailed description of harvesting material from filter membranes see our prior work (Ludwik et al., 2019, doi: 10.1016/j.ymeth.2019.02.002).Library Construction - 100 ng of total RNA obtained from either total cells or separated compartments was used for library preparation with the Truseq stranded total library prep kit (Illumina 20020596) according to the manufacturer’s recommendation.Sample Collection - hiPSCs with NGN2, ISL1, and LHX3 cassettes were differentiated into induced motor neurons (iMNs) using established protocols (Fernandopulle et al., 2018, doi: 10.1002/cpcb.51). RNA was collected in triplicates on days 1, 2, 4, 7, 14, and 21 of differentiation.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMarina ChekulaevafalseRNA-seq of differentiation stages of motor neuronsinduced motor neurons (iMNs) were differentiated from hiPSC line and different timepoints (day 1, 2, 4, 7, 14, 21) were subjected to RNA-seq2025-08-06T00:00:00Z2024-08-12T21:09:56.679Z2024-08-12T21:09:56.679ZE-MTAB-14359ERP163213EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184