biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsFazio GraziaNextSeq 550DNA-seqHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14362Pediatric patients affected by B-cell precursor ALL and enrolled in the AIEOP-BFM treatment protocol in Italian centers were analysed at diagnosis for mutations in cohesin genes. The experiment was performed designing a custom panel of 40 genes involved in leukaemia (IDT probe).biostudies-arrayexpressLibrary Construction - For library preparation we used the Illumina Nextera Flex for Enrichment protocolNucleic Acid Extraction - DNA was extracted from cell pellets using the Promega kit Wizard® SV Genomic DNA Purification SystemsSequencing - The NGS run has been performed on NexSeq550 Illumina platform, 2x150Sample Collection - Mononuclear cell by ficoll density purification of Bone Marrow or Peripheral BloodOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesFazio GraziafalsePediatric BCP-ALL analysis by DNA Target Capture NGS panelPediatric patients affected by B-cell precursor ALL and enrolled in the AIEOP-BFM treatment protocol in Italian centers were analysed at diagnosis for mutations in cohesin genes. The experiment was performed designing a custom panel of 40 genes involved in leukaemia (IDT probe).2025-08-31T00:00:00Z2025-08-31T01:02:25.47Z2024-08-13T11:59:43.42ZE-MTAB-14362ERP163229EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184