{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14364"],"description":["To compare PMBC scRNA-seq results of chronic (cases) vs transient (controls) ITP, including scTCR- and sc-BCR-seq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Steps were performed according to the 10X manufacturer protocol.","Nucleic Acid Extraction - PBMC samples were thawed in RPMI and 10% FCS and concentration was adjusted according to 10x genomics instructions. Cells were loaded in 5' Chromium Next GEM Single Cell Immune Profiling kits for gene expression v3.1. Human T and B cell Chromium Single Cell V(D)J Enrichment kits were used for targeted TCR and Ig enrichment. Steps were performed according to the manufacturer protocol.","Sequencing - Sequencing was performed on an Illumina Novaseq S4. The mean coverage was at least 40 000 reads per cell.","Sample Collection - Whole blood collected by venipuncture. PBMC isolated by ficoll and cryopreserved."],"figure_sub":["MINSEQE Score","Assays and Data","Processed Data","organisation","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - Raw sequencing data were mapped to the human transcriptome GRCh38 release 3.0.0. with 10X Genomics CellRanger version 3.0.","Data Transformation - Log-normalization. Data was scaled to produce standardized expression values for each gene across all cells (z-score transformation)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Homo sapiens"],"additional_accession":["ERP163250"],"pubmed_authors":["David Schmidt","Masja de Haas"]},"is_claimable":false,"name":"Single cell sequencing of PBMC from childhood ITP patients with transient or chronic disease course","description":"To compare PMBC scRNA-seq results of chronic (cases) vs transient (controls) ITP, including scTCR- and sc-BCR-seq.","dates":{"release":"2025-06-30T00:00:00Z","modification":"2026-05-27T16:30:28.44Z","creation":"2024-08-14T15:22:25.973Z"},"accession":"E-MTAB-14364","cross_references":{"ENA":["ERP163250"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}