biostudies-arrayexpressHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14364To compare PMBC scRNA-seq results of chronic (cases) vs transient (controls) ITP, including scTCR- and sc-BCR-seq.biostudies-arrayexpressLibrary Construction - Steps were performed according to the 10X manufacturer protocol.Nucleic Acid Extraction - PBMC samples were thawed in RPMI and 10% FCS and concentration was adjusted according to 10x genomics instructions. Cells were loaded in 5' Chromium Next GEM Single Cell Immune Profiling kits for gene expression v3.1. Human T and B cell Chromium Single Cell V(D)J Enrichment kits were used for targeted TCR and Ig enrichment. Steps were performed according to the manufacturer protocol.Sequencing - Sequencing was performed on an Illumina Novaseq S4. The mean coverage was at least 40 000 reads per cell.Sample Collection - Whole blood collected by venipuncture. PBMC isolated by ficoll and cryopreserved.MINSEQE ScoreAssays and DataProcessed DataorganisationMAGE-TAB FilesSequence Alignment - Raw sequencing data were mapped to the human transcriptome GRCh38 release 3.0.0. with 10X Genomics CellRanger version 3.0.Data Transformation - Log-normalization. Data was scaled to produce standardized expression values for each gene across all cells (z-score transformation).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsHomo sapiensERP163250David SchmidtMasja de HaasfalseSingle cell sequencing of PBMC from childhood ITP patients with transient or chronic disease courseTo compare PMBC scRNA-seq results of chronic (cases) vs transient (controls) ITP, including scTCR- and sc-BCR-seq.2025-06-30T00:00:00Z2026-05-27T16:30:28.44Z2024-08-14T15:22:25.973ZE-MTAB-14364ERP163250EFO_0002944EFO_0004170EFO_0005684EFO_0004917EFO_0005518EFO_0003816EFO_0004184