{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Yong Zhou"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14377"],"description":["We undertook RNA sequencing analysis using WT embryonic mouse hearts and Emp2-/- hearts at E10.5."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Paired-end sequencing of each RNA sequencing library was performed on an Illumina HiSeq.","Library Construction - Samples were processed using the Illumina mRNA-Seq Sample Preparation Kit as per the manufacturer's protocol","Nucleic Acid Extraction - RNA was extracted from the hearts of E10.5 WT and Emp2-/- mice using Trizol reagent (Life Technologies) in accordance with the manufacturer's instructions. The quality of the RNA was assessed using an Agilent 2100 Bioanalyzer.","Sample Collection - The hearts of E10.5 WT and Emp2-/- mice were collected."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - The raw reads in FASTQ format were aligned to the mouse genome using Tophat software."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq 4000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"pubmed_authors":["Yong Zhou"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of wild-type mice hearts and Emp2-/- hearts","description":"We undertook RNA sequencing analysis using WT embryonic mouse hearts and Emp2-/- hearts at E10.5.","dates":{"release":"2026-08-16T00:00:00Z","modification":"2026-08-16T01:00:50.17Z","creation":"2024-08-16T16:39:41.566Z"},"accession":"E-MTAB-14377","cross_references":{"ENA":["ERP163351"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}