{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Jeongwon Jeon"],"instrument_platform":["Illumina NovaSeq 6000","-"],"study_type":["RNA-seq of non coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14386"],"description":["Vascular aging refers to a series of processes where the elasticity of blood vessels diminishes, leading to stiffening, and deposition of fat components on the vessel walls, causing inflammation. Cardiovascular diseases, such as stroke and hypertension, play significant roles in morbidity and mortality rates among the elderly population. In this study, the Reactive Hyperemia Index (RHI) was measured to assess vascular endothelial function and aging-induced pathogenesis of vascular diseases in Korean subjects. We aimed to identify differentially expressed microRNAs (miRNAs) between groups of individuals at the ends of a continuum in vascular aging acceleration, revealing miRNAs regulating genes in endocrine hormone regulation and tumor-related pathways. We also discovered that the principal component characterizing the global miRNA expression profile is significantly associated with clinical traits including cholesterol levels."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - We used the data from the Namgaram project consisting of a total of 1,010 subjects at the age of 50 or above, recruited from the southern site (Jinju) in Korea, all the individuals were measured for age-related qualitative and quantitative traits, including age, grip strength, muscle strength score, and vascular endothelial functions. We randomly selected 3 samples from the top 10% and bottom 10% of RHI in each age group of 50s and 80s, and performed small RNA sequencing on a total of 12 samples.","Sequencing - smRNA sequencing was performed using the Illumina NovaSeq 6000 sequencing platform (Illumina, San Diego, CA, USA)","Nucleic Acid Extraction - Exosomes were isolated from serum using the Exo2D™-EV isolation kit for RNA analysis (for plasma/serurn). RNA was then isolated using the miRNeasy Serum/Plasma Advenced Kit (217204).","Library Construction - Individual libraries were generated SMARTer smRNA-Seq for Illumina Kit (Illumina, San Diego, CA, USA)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Jeongwon Jeon"],"additional_accession":[]},"is_claimable":false,"name":"Identification of Differentially Expressed miRNAs Involved in Vascular Aging and Association with the Endocrine Hormone Regulation Pathways","description":"Vascular aging refers to a series of processes where the elasticity of blood vessels diminishes, leading to stiffening, and deposition of fat components on the vessel walls, causing inflammation. Cardiovascular diseases, such as stroke and hypertension, play significant roles in morbidity and mortality rates among the elderly population. In this study, the Reactive Hyperemia Index (RHI) was measured to assess vascular endothelial function and aging-induced pathogenesis of vascular diseases in Korean subjects. We aimed to identify differentially expressed microRNAs (miRNAs) between groups of individuals at the ends of a continuum in vascular aging acceleration, revealing miRNAs regulating genes in endocrine hormone regulation and tumor-related pathways. We also discovered that the principal component characterizing the global miRNA expression profile is significantly associated with clinical traits including cholesterol levels.","dates":{"release":"2025-08-31T00:00:00Z","modification":"2025-08-31T01:02:29.712Z","creation":"2024-08-27T10:15:18.773Z"},"accession":"E-MTAB-14386","cross_references":{"ENA":["ERP163526"],"EFO":["EFO_0003737","EFO_0002944","EFO_0004170","EFO_0005518","EFO_0004184"]}}